The DBS-urine paired ELISA could be applied as an alternative to WB in HIV diagnosis, which would be valuable in resource-limited regions owing to the associated affordability and ease of use.ĮLISA HIV Substitutive strategy Western blot.Ĭopyright © 2016 The Authors. The kappa test indicated good conformity (kappa=0.954) between the two diagnostic strategies. For the remaining 945 subjects, both strategies indicated a negative result. In total, 18 subjects were diagnosed with possible HIV using the paired ELISA test, among whom, 11 subjects tested negative with WB, and one was confirmed to be HIV-positive during follow-up. The discordant case was judged HIV-positive during follow-up. The equivalence of the paired ELISA and WB strategies was assessed.Ī diagnosis of HIV was determined in 250 subjects according to the paired ELISA test, and in 249 according to the WB strategy. Two diagnostic testing strategies were conducted in parallel. Plasma, DBS, and urine samples were collected from 1213 subjects from different populations. The aim of this study was to evaluate the efficacy of a dry blood spots (DBS)-urine paired enzyme-linked immunosorbent assay (ELISA) test, instead of WB, for HIV antibody detection. However, this technique is time consuming and labor intensive, and its complexity restricts wide application in resource-limited regions. In China, western blot (WB) is the recommended procedure for the diagnosis of HIV infection. 8 National HIV/HCV Reference Laboratory, National Center for AIDS/STD Control and Prevention, China CDC, Beijing, China.7 Center for Disease Control and Prevention in Liangshan, Liangshan, China.6 Department of Clinical Laboratory, Beijing Youan Hospital, Capital Medical University, Beijing, China.5 Center for Disease Control and Prevention in Yunnan Province, Kunming, China.4 National HIV/HCV Reference Laboratory, National Center for AIDS/STD Control and Prevention, China CDC, Beijing, China.3 Department of Pathogenic Biology, Hebei Medical University, Shijiazhuang, China.2 Center for Disease Control and Prevention in Dehong, Dehong, China. As of November 2021, among the 42 states that cover routine HIV screening, 16 (CA, CO, DE, HI, KY, LA, MA, MT, NH, NJ, NV, NY, OH, OR, WA and WI) cover all USPTSF A and B graded.1 National HIV/HCV Reference Laboratory, National Center for AIDS/STD Control and Prevention, China CDC, Beijing, China Department of Clinical Laboratory, Beijing Youan Hospital, Capital Medical University, Beijing, China.
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